Purpose: The main purpose is to analyze the secretion sample collected around the tracheostomy site to detect the presence and characterize microorganisms, especially bacteria, fungi, or other pathogens.

Common indications: It is performed in patients with tracheostomies who present abnormal secretion, suspected infection, or to monitor the presence of microorganisms.

Sampling: A sample of the secretion around the tracheostomy is collected using a sterile method and with the appropriate equipment, such as a sterile swab or by a specific procedure recommended by the health professional.

Gram staining: The collected sample is subjected to the Gram staining technique, which involves the application of specific dyes to differentiate between gram-positive and gram-negative bacteria, as well as other microbial characteristics. This procedure allows the identification and classification of the microorganisms present.

Microscopy and analysis: After staining, microscopic examination of the stained sample is performed to observe the morphology, quantity, and type of microorganisms present around the tracheostomy site.

Interpretation of results: Microscopic observation allows the identification of the characteristics and quantity of microorganisms present in the sample, which is essential to determine whether there is an infection and, if so, the nature of the infection.

Follow-up: The results of this analysis are crucial for the treatment of infections or conditions related to tracheostomy. The information obtained guides the health professional in choosing the appropriate antibiotic treatment or other necessary therapeutic measures.